Meiji Techno TC5000 Series Bedienerhandbuch PDF herunterladen (Seite 26)

Possible Incident Light Fluorescence Operational Problems

If normal adjustments are not getting the results you expect, check these:

• Weak fluorescence or image intensity: specimens improperly stored or too old

• Rapid fading of specimens i.e.: photobleached

• Unspecific or erroneous filter combination

• Numerical aperture of objectives too low

• Eyepiece magnification too high

• Failing or old mercury lamp

• Ambient light or room too bright

• Secondary light due to reflection at condenser

• Low contrast image due to excitation bandwidth too wide

• Unspecified staining

• Fluorescing mounting medium

• Auto-fluorescence of objective or immersion oil

• Dirty optics

TC-5500 and TC-5600 Epi-Fluorescence Objectives

Epi-Fluorescent Objectives - Planachromat - Infinity Corrected - F = 200mm

MA849

TC Planachromat F4X objective ∞/1.0, NA: 0.13, WD = 17.5mm, F.L.= 50.0 (optional)

MA850

TC Planachromat F10X objective ∞/1.0, NA: 0.30, WD = 7.5mm, F.L.= 20.0 (included)

MA851

TC Planachromat F20X objective ∞/1.0, NA: 0.40, WD = 7.5mm, F.L.= 10.0 (included)

MA852

TC Planachromat F40X objective ∞/1.0, NA: 0.60, WD = 2.9mm, F.L.= 5.0 (optional)

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Meiji Techno TC5000 Series Bedienerhandbuch Seite 26