Meiji Techno TC5000 Series Bedienerhandbuch PDF herunterladen (Seite 23)

3.2 Transmitted Light Operation – Phase Contrast

Phase contrast is a very useful technique for high-contrast images of unstained or

transparent specimens in petri dishes, cell and tissue culture flasks and multi-well plates.

Meiji Techno’s TC5000 Series Phase Contrast Model features a centerable phase contrast

slider with 4X annulus, 10X-20X-40X annulus and an empty slot for brightfield.

A common phase annulus for the 10X–20X–40X objectives makes operation faster and

easier.

For improved contrast in 40X Phase Contrast, an optional NA 0.55

condenser part number MA854 is available as an option.

Setting Up Phase Contrast Mode

1. Install the phase contrast objectives into the nosepiece.

2. Select the proper phase slider setting to be used with

the proper objective.

3. To check to see if each phase annulus is centered,

remove the right hand eyepiece and insert the provided

focusing telescope.

4. Focus the telescope until the light and dark rings seen are in sharp focus.

5. The phase annuli are pre-aligned at the factory during

manufacturing. If, however, the annuli are not centered

as shown in Figure 3, use the supplied 2.5mm allen

wrench and adjust the two allen screws until the phase

annulus covers the light ring evenly. The location of the

phase annulus adjustment screws are shown in Figure4.

6. Remove the centering telescope and re-install the

eyepiece.

The microscope is now properly set for phase contrast observation mode.

Phase images will photograph and appear their best when the green interference filter

546nm (MA861) is placed into the light path since achromatic objectives are spherically

corrected for green light and the human eye picks up the green wavelength spectrum the

best.

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Meiji Techno TC5000 Series Bedienerhandbuch Seite 23