Meiji Techno MT5000 Series Bedienungsanleitung PDF herunterladen (Seite 14)

2.

Remove

the

right

hand

eyepiece

and insert

the

provided

centering telescope.

(Fig.2)

Then, rotate

the

10x

objective

into

place

along

with the

corresponding

10X-20X

condenser

annulus.

3.

Focus

the centering

telescope while

looking

through it

until

the light

and dark

rings

seen are

in

sharp

focus.

4. lf

the annuli

are

not

centered as shown

in Figure

3,

rotate

the knurled

annuli

adjustment

rings

on

the

bottom of

the

Zernlke

phase

condenser

with

your

fingers

to

adjust

the

phase

condenser annulus

with

each

phase

objective

as

shown at

right.

(Fig.a)

5.

Repeat the

process

for

the

rest

of

the

positions

being

sure to

match the annulus

to the right

objective.

6.

Remove the

centering

telescope

and

re-install

the eyepiece.

The microscope is now

properly

set

for

phase

contrast

observation

mode.

@@

Aligned Incorrect

Figure

3

o

Phase

images will

photograph

and

appear

their

best

when

the

green

interference filter

546nm

(MA861/05)

is

placed

into

the

light

path

since

achromatic objectives are spherically corrected

for

green

light

and the

human

eye

picks

up the

green

wavelength

spectrum best.

Possible Phase

Contrast

Mode

Operational

Problems

lf normal

adjustments are

not

getting

the results

you

expect, check

these:

.

Wrong

phase

annulus

being used.

.

Phase annulus not centered correctlv.

o

Wrong condenser installed.

r

Halos around the outlines ol delails are

optical

artifacts

which may obscure

details of

your

specimen.

This is a known limitation of

phase

contrast

microscopy.

r

Since

phase

annuli

limit

the

numerical

aperture of

the optical

system,

image resolution may

suffer

somewhat.

.

lf the specimen being observed

is too

thick,

phase

shifting will distort the image details.

centering

telescope

Figure 2

Knu rled

annuli

Figure 4

r3

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