
fi
Possible Brightfield Mode Operational
Problems
lf normal
adjustments are
not
getting
the results
you
expect,
check to see
if
these
conditions exist:
o
lncorrect condenser
/
objective combination being used
o
Incorrect components
inadvertently installed
o
Dirty or
smudged optics
MT5000 Series
U
Planachromat
Brightfield
Objectives
The following
are
the Plan
objectives that are standard
for the MT5000 Series
Brightfield Models:
Brightfield
Objectives
-
Planachromat
-
Infinity
Corrected
-
F
=
200mm
MA83O U
Planachromat 2.5X
objective,
NA:
0.07,
WD
=
5.7mm
(optional)
MA831 U Planachromat
4X
objective,
NA:
0.10,
WD
=
17.8mm
(included)
MA832 U Planachromat
10X
objective,
NA:
0.25,
WD
=
10.67mm
(included)
MA833
U
Planachromat 20X objective, NA:
0.40,
WD
=
7 .29mm
(optional)
MA834 U Planachromat
40X
objective,
NA:
0.65,
WD
=
0.5mm
(included)
MA835
U
Planachromat
50X objective,
NA:
0.87,
WD
=
0.28mm
(optional)
MA837
U
Planachromat 100X
oil
objective,
NA: 1
.25,
WD
=
0.23mm
(included)
3.3
Transmitted Light Operation
-
Phase Contrast
Phase contrast is
a
very
useful
technique tor high-contrast images oJ unstained or transparent specimens
mounted on
glass
slides. Phase
Contrast
models feature a
powerful
30W
halogen lamp for
extra
bright
phase
contrast
image
relief.
The MT5000
Series
Phase Contrast Models
come
standard with 10X, 20X,
40X
and
100X
U
Plan
Phase
objectives.
1.
Install
the
phase
contrast objectives
into the nosepiece
and
install
the Zerntke
Phase
Condenser
in
place
of
the
standard Abbe
Condenser by
lowering the
condenser
rack
and
loosening
the clamp screw shown at
right
and
then
sliding out the condenser.
(Fig.1)
condenser
clamp
screw
Figure 1
12
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